DNA

Part:BBa_K1039028

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-13)


PhiC31 Invertible Medium Strength Promoter Switch Test Construct

PhiC31 invertible promoter switch (BBa_K1039009) in PB state with gfp gene (BBa_K1039015) on the prefix side of the switch and rfp gene (BBa_J04450) on the suffix side. GFP is produced in this state of the switch.

The invertible promoter is strong promoter J23118.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 751
    Illegal NheI site found at 937
    Illegal NheI site found at 960
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1844
    Illegal AgeI site found at 1956
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 74


[edit]
Categories
Parameters
None